TABLE 1.
Oocyst recoveries from seeded water samples and CC-PCR results
| Sample | % Oocyst recovery with:
|
CC-PCR resultb | |
|---|---|---|---|
| Flotation-IFAa | IMS-IFAa | ||
| Raw water A | 18.3 | 56.6 | Positive |
| Raw water B | 14.9 | 33.7 | Positive |
| Raw water C | 14.2 | 10.4 | Positive |
| Raw water D | 22.3 | 6.9 | NA |
| Raw water E | 13.6 | 27.8 | Positive |
| Raw water F | 16.1 | 21.0 | Positive |
| Filter backwash water A | 9.4 | 17.2 | Positive |
| Filter backwash water B | <7.7 | <1.7 | Negative |
| Filter backwash water C | <3.9 | 6.2 | Positive |
| Filter backwash water D | 2.0 | 7.2 | NA |
| Filter backwash water E | <1.2 | 19.8 | Positive |
| Filter backwash water F | <10.9 | <2.9 | Negative |
| Deionized water negative controls (n = 8) | NDc | ND | Negative |
a
Mean ± standard deviation percent recoveries for raw water and filter backwash water samples were 16.6 ± 3.0 and 5.8 ± 3.7, respectively. Mean ± standard deviation percent recoveries for raw water and filter backwash water samples were 26.1 ± 16.5 and 9.1 ± 6.9, respectively.
b
Results showed 100% agreement with IFA results. NA, not available; CC-PCR assays were invalid due to a power failure during PCR (raw water sample D) and an incubator malfunction (filter backwash water sample D), respectively.
c
ND, None detected.