Figure 4. Splenectomy results in reduced monocyte-derived periplaque macrophages.

(A) Representative cortical sections from APP/PS1^{mTmG;Flt3-Cre} mice subjected to splenectomy at 5.5 months of age and sacrificed at 9.5 months of age, demonstrating fewer GFP⁺CD11b⁺ cells next to amyloid plaques stained with X-34. Areas boxed by dotted lines are shown at higher magnification on the right. (B) Density of GFP⁺ cells in the brain parenchyma and (C) quantitation of GFP⁺ cells as a fraction of CD11b⁺ plaque-associated cells in mice from A. (D) Abundance of CD11b⁺ cells adjacent to amyloid plaques stratified by size in mice from A. n = 6 after splenectomy; n = 5 after sham procedure. *P < 0.05, t test.