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. 2022 Jun 1;132(11):e151827. doi: 10.1172/JCI151827

Figure 1. Cd13-knockout (Cd13–/–) mice develop less inflammation compared with WT mice when injected with TNF-α.

Figure 1

TNF-α was injected into Cd13–/– and C57BL/6J WT mouse knee joints. PBS served as a negative control. (A) Cd13–/– mice developed significantly less knee swelling when TNF-α was injected into their knees compared with WT mice. n = number of knee joints per group: WT-PBS, n = 16; WT–TNF-α, n = 18; Cd13–/–-PBS, n = 16; Cd13–/––TNF-α, n = 10. (B) sCD13 was significantly elevated in TNF-α–injected knees (n = 8) in WT mice compared with PBS-injected mice (n = 7). (C) Knee homogenates from TNF-α–treated WT mice (n = 9) showed significantly higher MCP-1/CCL2 and IL-1β compared with TNF-α–treated Cd13–/– mice (n = 11). n = number of mouse knees per group. (D) TNF-α–treated WT mouse cryosections showed a marked increase in F4/80 staining compared with sections from PBS-treated mice, and this was not observed in the TNF-α–treated Cd13–/– mice. Red staining represents F4/80 for MNs/macrophages, while blue (DAPI) stains nucleus. Original magnification, ×200. Results are expressed as mean ± SD. *P < 0.05. Significance was determined by Kruskal-Wallis test (A and C), unpaired, 2-tailed Student’s t test (B), and 1-way ANOVA (C and D).