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. 2022 Jun 1;132(11):e154092. doi: 10.1172/JCI154092

Figure 3. TGF-β signaling in Osx-Cre–targeted mesenchymal stromal cells is essential for the development of myelofibrosis by Jak2V617F.

Figure 3

(A) Tgfb1 mRNA expression relative to β-actin mRNA in total bone marrow. (B) White blood cell (WBC) count, (C) red blood cell (RBC) count, (D) hematocrit (HCT), and (E) platelet (PLT) count and (F) spleen weight 5 months after tamoxifen-induced Jak2V617F expression. (G) Representative photomicrographs of femur sections stained for collagen I (yellow) or collagen III (red); nuclei were stained blue with DAPI. Scale bar: 20 μm. (H) Normalized fluorescence intensity for collagen I and III. Osx-TR2fl/–, Osx-Cre Tgfbr2fl/–. Data presented as the mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by Student’s t test (A) or 1-way ANOVA (B–E and G).