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. 2022 May 30;5:511. doi: 10.1038/s42003-022-03470-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Confocal images of OPC processes in control- and Pcdh15 shRNA treated cultures immunolabelled to detect microtubules (α-tubulin, green) and F-actin (Phalloidin, red). b Quantification of phalloidin intensity (integrated pixel density) in control and Pcdh15-knockdown OPCs (normalized to the average intensity of control OPC cultures) (n = 3 independent cultures, ≥51 veils analysed per condition; one-sample t-test for Pcdh15-shRNA: deviation from 100(%) = 30.25, SD of deviation = 9.19, t (2) = 5.697, p = 0.029). c Degree of colocalization between actin (phalloidin) and microtubule (α-actin) labelling in control and Pcdh15 knockdown OPC veils. Colocalization is expressed as a Pearson correlation coefficient (mean ± SEM, n = 3 independent cultures, ≥37 veils measured per condition; unpaired t-test, p = 0.81). d Protein lysates from control and Pcdh15 knockdown OPCs analysed by Western blot to detect Akt, phosphorylated Akt (p-Akt) and β-actin. e Expression of Akt in control and Pcdh15 knockdown OPC cultures, normalised to β-actin expression (integrated pixel density measured for each protein band; mean ± SEM, n = 3 independent cultures; unpaired t-test, p = 0.12). f Quantification of p-Akt (Ser473) and Akt in control and Pcdh15 knockdown OPCs, normalised to β-actin (integrated pixel density measured for each protein band; mean ± SEM, n = 3 independent cultures; unpaired t-test, p = 0.20). g Average fold change in cytoskeletal gene expression between OPCs transfected with control- and Pcdh15-shRNA (average from n = 3 independent cultures). h Protein lysates from control and Pcdh15 knockdown OPCs analysed by Western blot to detect CrkII and WAVE2, which are upstream of the Arp2/3 complex, Arp2, Arp3 and Arpc3, which are components of the Arp2/3 complex, and GAPDH. i CrkII, j WAVE2, k Arp2, l Arp3, and m Arpc3 protein expression was quantified by measuring the integrated pixel density of the corresponding Western blot band and normalising expression to GAPDH expression for each sample [mean ± SEM, n = 3 independent cultures, expression in control and Pcdh15 knockdown OPCs was compared by unpaired t-tests: i p = 0.039, j p = 0.004, k p = 0.0001, l p = 0.028, m p = 0.016]. Scale bars represent 5 µm.