Fig. 6. Sesquiterpenoid system in myriapods.
A Sesquiterpenoid pathway genes identified in sequenced myriapod genomes. B Summary diagram indicating different pathways that arthropod utilized for the synthesis of different sesquiterpenoid hormone. C The differential tissue expression of JHAMT in T. tuberculata (n = 3 biologically independent T. tuberculata). Data are presented as mean values +/− SEM. Individual data points are present on each bar. The vertical error bars indicate the standard error of mean (SEM) among the data points in each experiment. D The vitellogenin (Vg) expression in T. tuberculata fatbody (n = 5 biologically independent T. tuberculata fatbody tissue for 10−6 M FA in vitro treatment, n = 5 for 10−8 M FA treatment, n = 9 for 10−6 M MF treatment, n = 5 for 10−8 M MF treatment) post-hormonal treatment for 4 h. Data are presented as mean values +/− SEM. Individual data points are present on each bar. The vertical error bars indicate the standard error of mean (SEM) among the data points in each experiment. Two-tailed student t-test with two sample unequal variance test was conducted with confidence interval at 0.95. Red asterisk indicates the P value of the test, **≤0.05 and *≤0.08. The P value of 10−8 M FA experiment is 0.002915, while that of 10−6 M MF experiment is 0.07234 (as shown in the figure). E The Vg expression in H. holstii fatbody (n = 9 biologically independent H. holstii fatbody tissue for 10−6 M FA in vitro treatment, n = 6 for 10−8 M FA treatment, n = 6 for 10−6 M MF treatment, n = 7 for 10−8 M MF treatment) post-hormonal treatment for 4 h. Data are presented as mean values +/− SEM. Individual data points are present on each bar. The vertical error bars indicate the standard error of mean (SEM) among the data points in each experiment. Two-tailed student t-test with two sample unequal variance test was conducted with confidence interval at 0.95. F Summary indicating the evolution of sesquiterpenoid pathways in arthropods and cnidarians.