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. 1999 Aug;65(8):3449–3457. doi: 10.1128/aem.65.8.3449-3457.1999

TABLE 1.

Heat treatments applied to spores of nonproteolytic C. botulinum in a meat medium

Expt no. Heating temp (°C) Time (min)
Heat lethality (equivalent time [min] at target temp)d
Come-upa Target tempb Coolingc
1 70.0 8.2 100.6 7.8 104.9
2 70.0 7.0 527.0 7.5 529.1
1 70.0 12.7 989.6 5.8 998.9
1 70.0 12.8 1,587.9 6.2 1,596.3
2 70.0 14.5 2,056.0 12.5 2,065.9
2 70.0 14.0 2,537.5 7.5 2,544.5
2 75.0 9.5 280.0 8.0 284.6
1 75.0 11.8 457.4 7.1 463.1
2 75.0 9.0 730.0 7.0 734.2
2 75.0 12.5 1,065.5 5.0 1,071.5
1 75.0 15.8 1,373.7 5.7 1,376.5
2 75.0 11.0 1,785.0 12.0 1,793.0
1 80.0 10.0 7.8 4.7 11.4
2 80.0 13.5 64.5 5.0 69.7
1 80.0 15.3 93.7 6.8 98.0
1 80.0 15.3 123.7 5.3 127.9
2 80.0 14.5 178.0 10.0 183.8
1 80.0 10.1 226.1 5.7 229.6
2 80.0 12.5 289.0 8.5 294.9
2 80.0 15.0 355.0 8.0 362.7
2 85.0 9.6 18.7 6.9 23.3
1 85.0 14.8 28.0 9.1 35.7
2 85.0 11.0 46.6 6.3 52.0
1 85.0 11.5 53.5 5.1 57.8
2 85.0 10.8 78.4 5.5 83.8
1 90.0 11.6 5.8 4.5 10.3
2 90.0 9.3 6.4 5.7 10.9
2 90.0 11.9 10.1 5.4 15.3
2 90.0 12.7 17.3 4.3 23.5
2 90.0 11.9 27.8 5.9 33.5
a

Time required for the temperature at the center of the meat medium to reach within 0.1°C of the target maximum temperature. 

b

Time that the temperature at the center of the meat medium was within 0.1°C of the target maximum temperature. 

c

Time required for the temperature at the center of the meat medium to fall from within 0.1°C of the target maximum temperature to less than 10°C. 

d

Calculated as described by Fernández and Peck (10). See reference 10 for typical examples of heat treatment curves.