TABLE 3.

Effect of heat treatment and incubation temperature on time to growth from 10⁶ spores of nonproteolytic C. botulinum (types B, E, and F) in five subsequent replicate tubes of meat medium containing lysozyme (2,400 U/ml)

Heat treatment		Time (days) to growtha at the following incubation temp (°C)
°C	min	5	8	12	16	25
Unheated	0	14, 17, 17, 17, 17	7, 7, 7, 7, 7	5, 5, 5, 5, 5	2, 2, 2, 2, 2	1, 1, 1, 1, 1
70	104.9	14, 14, 16, 17, 17	9, 9, 9, 11, 11	6, 6, 6, 6, 6	2, 2, 2, 2, 3	1, 1, 1, 1, 1
	529.1	39, 39, 43, 43, 43	9, 9, 10, 10, 12	7, 7, 7, 7, 7	4, 4, 4, 4, 5	1, 1, 1, 1, 1
	998.9	48, 55, 60, 70, 87	17, 21, 24, 26, 26	8, 8, 9, 10, 11	3, 5, 6, 6, 6	2, 2, 2, 2, 2
	1,596.3	NGb	38, 45, 52, 54, 82	16, 16, 17, 17, 19	9, 9, 11, 11, 12	3, 3, 3, 3, 5
	2,065.9	NG	46, 53, 58, 76	16, 17, 22, 24, 27	8, 8, 9, 9, 10	3, 3, 3, 4, 4
	2,544.5	NG	NG	27, 29, 36, 38, 47	9, 10, 11, 12, 12	4, 4, 5, 5, 5
75	284.6	NG	33, 33, 33, 35, 35	13, 15, 18, 21, 21	11, 11, 11, 11, 11	4, 4, 4, 4, 5
	463.1	NG	NG	23, 25, 40, 40, 80	15, 25, 30, 30, 38	6, 8, 9, 10, 11
	734.2	NG	NG	21, 35, 54, 59	11, 18, 21, 21, 28	7, 8, 8, 12, 15
	1,071.5	NG	NG	63	63	10, 20, 22, 22, 29
	1,376.5	NG	NG	NG	38, 82	30, 34
	1,793.0	NG	NG	NG	56	48, 60, 69
80	11.4	46, 58, 65, 73, 73	24, 26, 26, 29, 29	10, 11, 14, 14, 15	8, 8, 8, 8, 8	4, 4, 4, 4, 5
	69.7	NG	26, 35, 39, 44, 50	21, 21, 26, 28, 33	8, 13, 14, 14, 14	4, 4, 5, 6, 6
	98.0	NG	42, 59, 73, 80	24, 31, 35, 38, 40	24, 24, 26, 29, 29	8, 11, 11, 11, 15
	127.9	NG	63, 77, 77, 87	40, 47, 47, 49, 56	17, 31, 31, 35, 38	11, 11, 11, 11, 12
	183.8	NG	86	62, 69	19, 24, 39, 41, 48	11, 13, 16, 16, 19
	229.6	NG	NG	80, 84	31, 45, 45, 47, 49	16, 17, 17, 19, 19
	294.9	NG	NG	NG	62, 65, 65, 76, 76	16, 23, 26, 28, 30
	362.7	NG	NG	NG	NG	19, 28, 28, 31, 37
85	23.3	NG	61, 64, 68, 68, 70	23, 30, 34, 34, 36	15, 18, 20, 20, 20	6, 6, 7, 7, 7
	35.7	NG	48, 53, 56, 56, 77	26, 31, 33, 33, 49	15, 23, 23, 23, 28	7, 9, 9, 9, 9
	52.0	NG	NG	46, 51, 51, 51, 54	23, 25, 25, 30, 32	8, 11, 11, 11, 11
	57.8	NG	87, 87, 89, 89, 89	37, 37, 49, 52, 54	33, 33, 33, 37, 40	9, 10, 10, 10, 12
	83.8	NG	NG	51, 64, 64, 65, 72	36, 38, 38, 40, 47	13, 15, 15, 15, 18
90	10.3	NG	54, 59, 70, 73, 75	38, 38, 38, 42, 42	24, 24, 26, 26, 26	8, 8, 8, 8, 8
	10.9	NG	58, 61, 61, 68, 68	30, 30, 32, 32, 32	18, 18, 18, 20, 20	6, 7, 7, 7, 7
	15.3	NG	68, 72, 75, 75	34, 36, 38, 42, 47	23, 23, 23, 25, 25	8, 9, 9, 9, 11
	23.5	NG	79	47, 47, 47, 54, 56	23, 27, 27, 27, 30	9, 11, 11, 11, 11
	33.5	NG	NG	56, 56, 56, 61, 65	23, 30, 34, 38, 38	14, 14, 14, 14, 17

^aAs determined visually. When fewer than five values are included, the rest of the tubes did not show visible grow after 90 days. 

^bNG, no growth.