TABLE 4.

Comparison of the results of previous studies of the effect of heat treatment and subsequent incubation temperature on growth from spores of nonproteolytic C. botulinum, in the presence of lysozyme, with results predicted from the present model

Heat treatment		Incubation temp (°C)	Lysozyme activity (U/ml)a	Time to growth (days)		Reference
°C	min			Observedb	Predicted
80	20	5	1,200	53	68	21a
		8	1,200	24	38	21a
		12	1,200	16	20	21a
		16	1,200	6	12	21a
80	23	6	625	40	56	27
		8	625	23	39	27
		10	625	19	28	27
		12	625	12	20	27
		25	625	3	5	27
90	19.8	8	2,400	68	67	26
		12	2,400	31	36	26
		16	2,400	24	21	26
		25	2,400	9	10	26
90	20	8	1,200	87	67	13
		12	1,200	49	36	13
		16	1,200	29	21	13

^aPrior to heating. 

^bWhen 10⁶ spores of a mixture of strains of nonproteolytic C. botulinum were inoculated in 20 ml of meat medium containing lysozyme, given the indicated heat treatment, and subsequently incubated at the indicated temperature.