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. 1999 Aug;65(8):3449–3457. doi: 10.1128/aem.65.8.3449-3457.1999

TABLE 5.

Assessment of the ability of recommended treatments for REPFEDs to give a 6D reduction in the probability of growth from spores of nonproteolytic C. botulinuma

Organization (reference) Recommended heat treatment
6D reduction by heat treatment alone with lysozyme:
6D process by heat treatment and subsequent storage at the following temp (°C) for 42 days:
8
12
°C min Absent Presentb Lysozyme absent Lysozyme presentb Lysozyme absent Lysozyme presentb
ACMSF (1) 70 1,675 No No No No No No
75 464 No No Yes Yes No No
80 129 No No Yes Yes No No
85 36 Yes No Yes Yes Yes No
90 10 Yes No Yes Yes Yes No
ECFF (9) 80 270 No No Yes Yes Yes Yes
85 52 Yes No Yes Yes Yes No
90 10 Yes No Yes Yes Yes No
a

Results are from this study and reference 10

b

Lysozyme was present at 2,400 U/ml prior to heating.