Table 3.

Primer sequences and characteristics

HBV S area		Nucleic acid position	Primer sequences (5′–3′) (bp)	Amplification length
Outer primers	P1	300–321	5′-CATCTTCTTGTTGGTTCTTCTG-3′	417 bp
	P2	715–695	5′-TTAGGGTTTAAATGTATACCC-3′
Inner primers	P3	421–441	5′-TCTATGTTTCCCTCTTGTTGC-3′	206 bp
	P4	626–605	5′-TACCACATCATCCATATAACTG-3′