Table 2.

The experimental runs of CCD for optimization of protease production

Run order	Factor					Blocks
	X1	X2	X3	X4	X5
1	48	35	4	50	1.0	1
2	96	35	4	50	2.0	1
3	48	45	4	50	2.0	1
4	96	45	4	50	1.0	1
5	48	35	6	50	2.0	1
6	96	35	6	50	1.0	1
7	48	45	6	50	1.0	1
8	96	45	6	50	2.0	1
9	48	35	4	70	2.0	1
10	96	35	4	70	1.0	1
11	48	45	4	70	1.0	1
12	96	45	4	70	2.0	1
13	48	35	6	70	1.0	1
14	96	35	6	70	2.0	1
15	48	45	6	70	2.0	1
16	96	45	6	70	1.0	1
17	48	35	4	50	2.0	1
18	96	35	4	50	1.0	1
19	48	45	4	50	1.0	1
20	96	45	4	50	2.0	1
21	48	35	6	50	1.0	1
22	96	35	6	50	2.0	1
23	48	45	6	50	2.0	1
24	96	45	6	50	1.0	1
25	48	35	4	70	1.0	1
26	96	35	4	70	2.0	1
27	48	45	4	70	2.0	1
28	96	45	4	70	1.0	1
29	48	35	6	70	2.0	1
30	96	35	6	70	1.0	1
31	48	45	6	70	1.0	1
32	96	45	6	70	2.0	1
33	72	40	5	60	1.5	1
34	72	40	5	60	1.5	1
35	72	40	5	60	1.5	1
36	72	40	5	60	1.5	1
37	72	40	5	60	1.5	1
38	24	40	5	60	1.5	2
39	120	40	5	60	1.5	2
40	72	30	5	60	1.5	2
41	72	50	5	60	1.5	2
42	72	40	3	60	1.5	2
43	72	40	7	60	1.5	2
44	72	40	5	40	1.5	2
45	72	40	5	80	1.5	2
46	72	40	5	60	0.5	2
47	72	40	5	60	2.5	2