. 2022 May 30;20:82. doi: 10.1186/s43141-022-00358-9

Table 4.

The effect of nitrogen source on protease production by Rhizomucor miehei

Type and concentration of nitrogen source	Protein content (mg/mL)	Milk clotting activity (SU/mL)	Specific activity (SU/mg)	Proteolytic activity (PU/mL)	MCA/PA
Blank	2.11 ± 0.01^ab	444 ± 19.50^a	210.43 ± 7.96^a	0.92 ± 0.06^a	481.7 ± 11.27^a
Yeast extract
1%	2.11 ± 0.02^a	555.42 ± 23.43^b	263.23 ± 8.65^b	0.94 ± 0.06^a	591.45 ± 14.46^b
2%	2.19 ± 0.04^bcd	424.14 ± 15.61^a	193.67 ± 4.03^ac	0.94 ± 0.06^a	450.42 ± 19.29^ac
3%	2.13 ± 0.04^ab	370.28 ± 9.39^c	173.84 ± 1.88^c	1.33 ± 0.07^b	279.48 ± 8.03^d
4%	2.13 ± 0.05^ab	324.32 ± 22.60^d	152.26 ± 7.82^d	1.38 ± 0.08^b	243.92 ± 5.81^e
5%	2.10 ± 0.04^a	231.75 ± 20.41^e	110.36 ± 7.83^e	1.41 ± 0.10^b	160.89 ± 7.40^f
Peptone
1%	2.11 ± 0.03^ab	571.42 ± 19.51^b	270.82 ± 7.09^bf	1.46 ± 0.10^b	392.04 ± 15.20^g
2%	2.13 ± 0.03^ab	648.64 ± 27.44^fg	304.53 ± 8.61^gh	2.34 ± 0.10^dc	278.75 ± 14.31^d
3%	2.14 ± 0.04^ab	666.66 ± 24.57^f	311.52 ± 6.72^g	2.46 ± 0.20^d	272.23 ± 24.91^d
4%	2.12 ± 0.04^ab	613.89 ± 20.42^g	289.57 ± 4.87^fhkm	1.92 ± 0.30^e	320.51 ± 15.35^h
5%	2.08±0.04^a	571.42 ± 18.35^b	274.72 ± 3.90^bk	1.30 ± 0.16^b	438.79 ± 9.88^c
Casein
1%	2.23±0.04^c	827.58 ± 20.25^h	371.11 ± 3.59ⁿ	1.40 ± 0.07^b	591.46 ± 23.51^b
2%	2.27±0.06^c	857.14 ± 20.81^h	377.59 ± 1.91ⁿ	1.62 ± 0.09^be	527.76 ± 20.62ⁱ
3%	2.38±0.05^e	666.66 ± 23.61^f	280.11 ± 4.63^g	2.02 ± 0.10^ce	328.87 ± 11.84^h
4%	2.24±0.03^cd	666.66 ± 19.11^f	297.62 ± 4.25^g	5.26 ± 0.27^f	127.27 ± 3.96^j
5%	2.15±0.03^ad	648.64 ± 19.42^fg	301.69 ± 4.95^gm	5.98 ± 0.32^g	108.62 ± 2.49^j

The results are expressed as mean ± SD. Within columns, values followed by the same letter(s) are not significantly different at the 0.01 level