FIG. 5.

Toxicity of GST fusion proteins of the processed fragments of Cry4A. The GST-Cry4A fusion proteins were adsorbed to the latex beads to give a final protein concentration of 0.1 μg/ml. The mosquitocidal activities were assayed on the 4th instar larvae of C. pipiens. Before the assays, each larva was transferred to 200 μl of distilled water in each well of a 96-well plate. After 8 h, the GST-Cry4A fusion proteins adsorbed to latex beads were added. In each experiment, 96 larvae were tested at the protein concentration of 0.5 μg/ml, and the assay was performed more than three times. The mortality of mosquito larvae in the presence of each GST fusion protein was scored after a 12-h incubation at 25°C by subtracting the mortality with latex beads alone. The error bars denote standard errors of the mean. (A) The relative mortalities with GST-Cry4A fusion proteins are expressed as a proportion of the mortality with GST-20 and GST-45. (B) The mortality with GST-60 and GST-60RQA is shown.