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The effects of MSU-induced neutrophil-derived exosomes on hFOB. (A) CCK-8 assay is used to detect the cell viability. (B) Apoptosis is detected by flow cytometry. (C) QPCR was used to assess miRNA expression levels of ALP, OPG and RANKL. (D) Protein expression levels of ALP, OPG and RANKL in hFOB cultures were determined via western blot analysis. P<0.01**, P<0.001 ***determined by the t-test. ns, no significance.
