Figure 9.
The effect of neutrophil-derived exosomes stimulated by MSU on viability of hFOB (A) Transfection of hFOB with miRNA inhibitors. Interfering fragments are verified by qPCR. (B) CCK8 assay to exam the cell viability. (C) Flow cytometric analyses to detect the percentages of apoptotic cells. (D) QPCR to detect the expression levels of ALP, OPG, and RANKL. (E) Western blot analysis to determine the expression levels of ALP, OPG, and RANKL (P < 0.05 *, P < 0.01 **, P < 0.001 ***).