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. 2022 May 17;16:843303. doi: 10.3389/fnana.2022.843303

FIGURE 2.

FIGURE 2

Monosynaptic afferent from neuromodulator neurons. (A) Immunohistochemical staining of input from LDT to PPN. (B) A three-panel presentation with RV, anti-ChAT, and overlay; arrows point out ChAT-positive input neurons. (C) Proportion of ChAT+ neurons input from LDT to PPN. (D) Immunohistochemical staining of input from PPN to LDT. (E) A three-panel presentation with RV, anti-ChAT, and overlay; arrows point out ChAT-positive input neurons. (F) Proportion of ChAT-positive neurons input from PPN to LDT. (G) Immunohistochemical staining of input neurons in the DR. (H) A three-panel presentation with RV, anti-TPH2, and overlay; arrows point out TPH2-positive input neurons. (I) Proportion of TPH2+ neurons input from DR to PPN or LDT. (J) Immunohistochemical staining of input neurons in the LC. (K) A three-panel presentation with RV, anti-TH, and overlay; arrows point out TH-positive input neurons. (L) Proportion of TPH2+ neurons input from DR to PPN or LDT. (M,N) Immunohistochemical staining of input neurons in the VTA labeled with RV, CTB, and FG, respectively. (O) The interconnections between PMT cholinergic neurons and neuromodulatory nuclei. The abbreviations of nuclei suffixed with “-c” meant nuclei in the contralateral. A scale bar − (A,D,G,J,M,N) 200 μm; (B,E,H,K) 50 μm. Data are shown as mean ± SEM. PPN = LDT = 4 mice.