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Purification of S. cerevisiae esterase
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
| Preparation of cell-free crude extract | 61 | 5.4 | 0.09 | 1 | 100 |
| (NH4)2SO4 fractionation | 17 | 4.4 | 0.26 | 2.9 | 81 |
| Phenyl Sepharose HR chromatography | 4.2 | 3.4 | 0.8 | 8.9 | 63 |
| Q Sepharose fast flow chromatography | 1.1 | 2.9 | 2.6 | 29 | 54 |
| Sephacryl HR200 chromatography | 0.17 | 1.2 | 7 | 78 | 22 |
| Chromatofocusing | 0.022 | 0.24 | 11 | 120 | 4.4 |
