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. 2022 May 17;12:858379. doi: 10.3389/fonc.2022.858379

Figure 3.

Figure 3

Metabolic effect of transient silencing of inducible expression of PD-L1 in A498 and 786-O cells. (A) 24 hours after silencing of PD-L1 (PD-L1 siRNA) or scrambled siRNA used as controls (Cn siRNA), A498 and 786-O cells were treated with IFNγ (50 ng/ml) for 24 hours, n=3. The protein expression of PD-L1, PD-L2, JAK2, STAT1 and the phosphorylation of STAT1 was then assessed by immunoblotting. (B) the metabolic effect of PD-L1 silencing was assessed using a bioanalyzer in IFNγ treated and PD-L1 silenced A498 and 786-O cells. A498 and 786-O cells without IFNγ treatment are used as controls. ECAR and OCR data are presented as mean ± SD (n=3) *p<0.05, **p<0.001. (C, D) A decrease in glucose and glutamine consumption was observed in PD-L1 silenced (PD-L1si IFNγ) A498 and 786-O cells as compared to IFNγ treated (Cnsi IFNγ) treated cells. The data are presented as mean ± SD (n=3) *p<0.05, **p<0.001, #p<0.05 for CN IFNγ compared to CN.