Figure 4.

Transcriptional effects of IFNγ treatment with or without PD-L1 silencing. (A) Volcano plots depicting the number of genes upregulated and downregulated in A498 and 786-O cells with PD-L1 expression (Cnsi_IFNγ vs Cnsi) and with silenced PD-L1 expression (PD-L1si_IFNγ vs Cnsi_IFNγ) (n=3, for each group). The GSEA analysis indicating upregulated signaling pathways with IFNγ treatement in A498 and 786-O cells is shown in the table below the volcano plots, “-”, data not determined; RNA-Seq differential expression of genes (DEG) in A498 and 786-O cells with high PD-L1 expression (Cnsi_IFNγ vs Cnsi) are shown in (B) and with silenced PD-L1 expression (PD-L1si_IFNγ vs Cnsi_IFNγ) are shown in (C), “-”, data not determined. (D) illustration of the effect of IFNγ on tryptophan metabolism; (E) Genes regulated by IFNγ in a PD-L1 dependent manner.