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. 2022 May 17;14:889983. doi: 10.3389/fnagi.2022.889983

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Copyright © 2022 Stekic, Zeljkovic, Zaric Kontic, Mihajlovic, Adzic, Stevanovic, Ninkovic, Grkovic, Ilic, Nedeljkovic and Dragic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of iTBS treatment on TMT-induced astrocytic inflammation. (A) Double immunofluorescence staining directed to GFAP (red) and IL-1β (green) of coronal hippocampal sections captured in the same region levels. Scale bar = 50 μm. Integration of immunofluorescence intensity and quantitative analyses showing (B) Pearson’s correlation coefficient (PCC), (C) number of GFAP⁺/IL-1β⁺ cells in high power field (HPF), and (D) fluorescence intensity of IL-1β in arbitrary units (AU). Bars represent the mean value ± SEM of specific parameters calculated from acquired images (10–16/animal). Results of post hoc Tukey’s test and significance shown inside graphs: *p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, ns, no significance.