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. 2022 May 17;14:889983. doi: 10.3389/fnagi.2022.889983

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Copyright © 2022 Stekic, Zeljkovic, Zaric Kontic, Mihajlovic, Adzic, Stevanovic, Ninkovic, Grkovic, Ilic, Nedeljkovic and Dragic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of iTBS treatment on TMT-attenuated PI3K/Akt/mTOR signaling. Representative support membrane and quantitative data of Western blot analysis showing relative protein abundance of (A) PI3K (p85) or phosphorylated/non-phosphorylated (B) p-Akt/t-Akt ratio, (C) p-mTOR/t-mTOR, (D) p-ERK/t-ERK ratio in the hippocampal cytosol. Bars represent mean value of target protein (A) or ratio of phosphorylated and non-phosphorylated forms (B–D) normalized to β-actin abundance ± SEM (from n = 4–5 individual animals, in 2–4 technical replicates), expressed relative to control (arbitrary defined as 100%). Results of post hoc Tukey’s test and significance are shown inside graphs: *p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001, ns, no significance. Dots in the graphs represent values of individual animals.