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. 2022 May 17;13:883057. doi: 10.3389/fphar.2022.883057

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Copyright © 2022 Yi, Yan, Guo, Liu, Wang, Shang, Wei, Cui, Sun, Ren and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PINK1/Parkin-mediated mitophagy was involved in the protective effects of EPO on mesangial cells exposed to HG. Representative immunoblots and quantification of (A) PINK1 and (B) Parkin. Immunofluorescence images of colocalization of (C) LC3 with mitochondria, (D) Parkin with mitochondria and (E) LC3 with Parkin as well as the (F) colocalization of GFP-LC3 with mitochondria. Values are presented as mean ± SEM, n = 3. *p < 0.05 vs. Control group; ^# p < 0.05 vs. HG group.