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. 2022 May 18;9:792717. doi: 10.3389/fcvm.2022.792717

FIGURE 3.

FIGURE 3

OxLDL induces CD36-Vimentin colocalization in early endosome. (A) Representative immunocytochemistry images of wild type macrophages incubated with or without oxLDL (50 μg/ml) for 10 min. Cells were stained with a rabbit polyclonal antibody to CD36 (Dylight 594; Red) and a mouse monoclonal antibody to vimentin (Alexa 488; Green). Scale Bars represent 20 μm. (B) Vim +/+ mouse peritoneal macrophages were treated with or without oxLDL (50 μg/ml) for 10 min and immunoprecipitated with indicated antibodies. The precipitants were analyzed by western blot with antibodies against vimentin and CD36. (C) Sucrose gradient early endosome fraction test. Wild type macrophages were incubated with or without oxLDL (50 μg/ml) for 10 min and homogenized. The lysates were added to 15∼40% sucrose gradient column and centrifuged. (a) oxLDL-untreated and (b) oxLDL-treated macrophage lysates. Immunoblot analyses for indicated proteins.