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. 2022 May 18;9:792717. doi: 10.3389/fcvm.2022.792717

FIGURE 6.

FIGURE 6

Vimentin (Ser72) phosphorylation induced by oxLDL is required for the uptake of oxLDL. (A) Flow cytometry. Fluorescence intensities of diI-oxLDL-treated Vim−/– BMDM. Cells were transfected with vectors for vimentin or vimentin mutants. (B) Flow cytometry. Geometric mean fluorescence intensities of diI-oxLDL-treated SW13 cells. Cells were transfected with vectors for vimentin or vimentin mutants. (C) Measurement of membrane CD36. Vim−/– BMDM were transfected with vimentin or mutant vimentin vectors. Stabilized macrophages were stained with APC conjugated anti-CD36 antibody and analyzed using flow cytometry. (D) The representative immunohistochemistry images of aortic sinuses of Ldlr−/– mice fed a normal diet for 14 weeks or western diet for 14 weeks. Both samples were stained with antibody against phosphorylated vimentin (Ser72) (Dylight 594; Red) and antibody against f4/80 (Alexa 488; Green), a macrophage marker. Scale bars represent 1 μm. N.S. not significant, ***p < 0.001.