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. 2022 May 17;13:868472. doi: 10.3389/fpls.2022.868472

TABLE 2.

The information of qPCR primers.

Gene name Primer Tm Length Product length
CHLP-F ACGTCTCACCTGATTTCTACGG 59.84 22 85
CHLP-R TCTGATTTGTGGGTGACGGTG 60.54 21
ACS1-F TGGTTTAGGGTTTGCTTCGC 59.04 20 148
ACS2-R TCAACTTCAGCCCAGTGTTCT 59.51 21
CYP75B2-F GTGGGGAGGAGAGTGTTTGG 60.0 20 116
CYP75B3-R CCAAGCCCAGGCACAAAATC 60.0 20
ADC1-F GGATGTCCTGCGAGCGATG 60.95 19 82
ADC2-R ACTGTGAGCGTATTCCTCGG 59.54 20
CYP74B2-F GCTAGGCTTCAACGCATTCG 60.0 20 92
CYP74B3-R GTCTCTGCTTCAGCCCTGTT 60.0 20
ACS2-F GTGAAGCTTAACGTGTCGCC 59.8 20 115
ACS3-R TTATTCTCCTCAGCGCCACC 59.8 20
actin-F AATCCCAAGGCAAACAGA 51.0 18
actin-R CACCATCACCAGAATCCAG 53.0 19