Gel electrophoresis of PCR products from T. cruzi isolates. (a) 1·5% Agarose gel electrophoresis of SL-IR amplicons. (b) 12% Acrylamide gel electrophoresis of 24Sα amplicons. Lane M, 100 bp DNA ladder (hyperladder IV, Bioline, UK); lanes 1–3, isolates 1593, 1595 and 1598 from patients infected in the Caracas outbreak; lane 4, laboratory strain PM; lane 5, isolate AC1 from an infected Panstrongylus geniculatus triatomine captured at the guava juice preparation site; lane 6, isolate RATA 6 from an infected rat captured at the guava juice preparation site; lanes C1, C2, C3, T. cruzi control strains Ikiakora (DTU TcIII), Munanta (DTU TcI) and Guateque (DTU TcVI).