Fig 1.

(A) Timeline of Morris water maze (MWM) training and assesment, diet manipulations and administration of cyclophosphamide (CYP), doxorubicin (DOX) or 0.9% saline. Female BALB/C mice (N=30) were trained in the MWM for 5 days (days 10–14). Weekly assessments were performed on days 15–71, with trials 1, 2, 4 and 5 occurring with the platform present, and trial 3 serving as a probe trial (platform removed) to assess spatial memory. Following baseline assessment on day 15, mice either remained on standard diet or were switched to a 2% choline diet. CYP (25mg/kg) and DOX (2.5mg/kg) were administered (i.v.) on days 16, 23, 30 and 37 to mice on standard (n=10) or 2% choline (n=10) diet. A separate group of mice on standard diet (n=10) received injections (i.v.) of saline (0.9%) on days 16, 23, 30 and 37 and were assessed cuncurrently. (B) The weights (g) of female BALB/C mice (N=30) were assessed weekly throughout the study. Data are presented as group means +/− S.E.M. Using Dunnett’s test, signifcant (p<0.05) differences from baseline (day 9) weights are indicated with a *. Using Tukey’s HSD, signifcant (p<0.05) differences from the Standard Diet – Saline group are indicated with † and differences from Choline Diet (2%) – DOX+CYP group are indicated with •.