KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit Anti-Syt7 | Synaptic Systems | Synaptic Systems Cat# 105 173; RRID:AB_887838 |
| Mouse Anti-Syt7 N275-14 | Neuromab/Sigma | Millipore Cat# MABN665; RRID:AB_2888943 |
| Guinea Pig Anti-vesicular glutamate transporter 2 (VGLUT2) | Synaptic Systems | Synaptic Systems Cat# 135 404; RRID:AB_887884 |
| Anti-rabbit Alexa Fluor 647 | Abcam | Abcam Cat# ab150083; RRID:AB_2714032 |
| Anti-guinea-pig Alexa Fluor 488 | Abcam | Abcam Cat#ab150185; RRID:AB_2736871 |
| Anti-mouse Alexa Fluor 647 | Abcam | Abcam Cat#ab150115; RRID:AB_2687948 |
| Anti-Guinea-pig Alexa Fluor 555 | Abcam | Abcam Cat#ab150186 |
| Goat-anti-GFP FITC | Abcam | Abcam Cat# ab6662; RRID:AB_305635 |
| Chemicals, peptides, and recombinant proteins | ||
| NBQX disodium salt | Abcam | ab120046 |
| Picrotoxin | Abcam | ab120315 |
| (+)-Bicuculline | Sigma | 14340 |
| QX-314 Cl | Abcam | ab120118 |
| Experimental models: organisms/strains | ||
| C57BL/6, Mus musculus | Charles River | N/A |
| CD-1 IGS, Mus musculus | Charles River | N/A |
| Syt7 Global Knockout B6.129S1-Syt7tm 1Nan/J | Jackson Labs | IMSR Cat# JAX:004950; RRID:IMSR_JAX:004950 |
| Oligonucleotides | ||
| RNAscope® Probe - Mm-Syt7-C2 | ACD-Bio | 546201-C2 |
| RNAscope® Probe - Mm-Slc17a7-C3 | ACD-Bio | 416631-C3 |
| RNAscope® Probe - Mm-Slc17a6-C3 | ACD-Bio | 319171-C3 |
| Recombinant DNA | ||
| AAV9-hSyn-hChR2(H134R)-EYFP | University of Pennsylvania Vector Core /Addgene | 26973-AAV9 |
| AAV9-hSyn-hChR2(H134R)-EYFP-P2A-Syt7WT | Boston Children’s Vector Core | N/A |
| Software and algorithms | ||
| MATLAB | Mathworks | RRID:SCR_001622 |
| IgorPro | Wavemetrics | RRID:SCR_000325 |
| ImageJ/Fiji software | NIH/Fiji | RRID: SCR_002285 |
| GraphPad Prism 7 | GraphPad | RRID: SCR 002798 |
| FluoView software | Olympus | RRID:SCR_014215 |
| OlyVIA | Olympus | RRID:SCR_016167 |
| Microsoft Excel | Microsoft | RRID:SCR_016137 |
| MultiClamp 700B Commander | Molecular Devices / Axon Instruments | RRID:SCR_018455 |
| Adobe Illustrator | Adobe | RRID:SCR_010279 |
| Action bar for ImageJ | By Jérôme Mutterer; “Custom toolbars and mini applications with Action Bar.” | https://figshare.com/articles/dataset/Custom_toolbars_and_mini_applications_with_Action_Bar/3397603/3 |
| VSI Reader Actionbar (for ImageJ) | By Olivier Burri, Romain Guiet, Arne Seitz via Biolmaging & Optics Platform (BIOP) of the Ecole polytechnique fédérale de Lausanne (EPFL) | https://www.epfl.ch/research/facilities/ptbiop/ |