Figure 4.

MV4;11 cells overexpressing WT or gain of function S159A mutant MCL-1 are resistant to ABT-199 but are similarly sensitive to ONO-7475 compared to control cells. MV4;11 control (Empty Vector; EV) and MV4;11 cells overexpressing WT MCL-1 or S159A mutant MCL-1 were treated with vehicle (0.1% DMSO), 10 nM, or 50 nM ONO-7475 for 72 hours. One-way ANOVA with Dunnett’s posttest was performed to determine significance (^*P<0.033; ^**P<0.002; ^***P<0.001) In (A) western blot of MCL-1 expression levels in EV control, WT MCL-1 OE, and S159A mutant MCL-1 OE are depicted. Viable total cells (B) and percentage of apoptotic cells (C) were determined by flow cytometry using counting beads, Annexin V, and DAPI. (D) AXL expression in MV4;11 cells based on MCL-1 expression levels. Densitometry was performed by normalizing to tubulin using ImageJ.