Figure 5.

Neutrophil-targeted ROS inhibition blocks NETs and thrombosis in whole blood. Neutrophil-depleted blood was reconstituted with autologous neutrophils pretreated with vehicle control or DPI, then treated with normal IgG or HIT IgG plus heparin. (A) Representative fluorescent images showing extracellular DNA (Sytox, blue), neutrophils (anti-CD15 AF594, green), and platelets (anti-CD41 V450, red). Quantification of area coverage of (B) DNA, (C) neutrophils, and (D) platelets under different treatment conditions. Scale bar, 50 µm. Data expressed as mean plus or minus SD (n = 3). *P < .05; ns, not significant, by 1-way ANOVA with Tukey correction for multiple comparisons.