Figure 1: Phenotypic Variation in Host Infection Outcomes Correlates with Changes in Host Energy Substrate Resource Allocation.

(A) Systemic induction of Dro-GFP after P.e. oral infection.

(B) Ratios of Dro-GFP flies after P.e. oral infection at 8hrs and 20hrs; n=400.

(C-D) RNA-seq. transcriptomics analysis of NF-kB/Relish target genes in dissected (C) thoraces or (D) carcass (adipose) of Dro-GFP flies.

(E-F) Infection resistance of Dro-GFP flies; (E) pathogen abundance (CFUs) at 4hrs, 8hrs and 20hrs; n=12, (F) survival rates; n=300.

(G-H) RNA-seq. transcriptomics analysis of stress responses genes in dissected (G) thoraces or (H) carcass of Dro-GFP flies.

(I) Measurement of defecation in Dro-GFP flies after P.e. oral infection; n=260.

(J-L) Changes in systemic lipid metabolism of Dro-GFP flies. (J) Neutral lipid storage; Oil Red O (ORO) stain and Nile-Red stain (lipid droplets) were assayed in dissected carcass/fat body; (K) Total TAG levels of whole flies and (L) Circulating lipids levels in isolated hemolymph; n=4.

(M-N) Changes in systemic lipid metabolism in PplGal4, tubGal80ts>UAS-Apo-ltp^RNAi or UAS-Apo-lpp^RNAi flies. (M) Total TAG levels of whole flies; n=3, and (N) ORO stain of neutral lipid storage.

(O-P) Infection resistance. (O) Measurement of defecation; n=200. (P) Measurement of CFUs per midgut; n=12.

Error bars represent mean±SE, *P<0.01.