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. Author manuscript; available in PMC: 2022 Dec 7.
Published in final edited form as: Cell Metab. 2021 Oct 27;33(12):2428–2444.e8. doi: 10.1016/j.cmet.2021.10.003

Figure 6: Muscle-derived Glutamate Adjusts Smvt-mediated Vitamin Metabolism in Adipose to Shape Phenotypic Diversity of Host Infection Outcomes.

Figure 6:

(A) RNA-seq. transcriptomics analysis (dissected carcass/fat body) of Act88FGal4>UAS-RelishRNAi#1 flies after P.e. oral infection (±P.e.).

(B-C) Unique up-regulated genes analyzed for Gene Ontology (GO) Terms and Pathway Enrichment (enrichment P<0.001).

(D) Fold change (RelishRNAi#1/Ctrl.) of select genes involved in cellular transport and co-factor biology.

(E) Model depicting the function of Smvt in transport and uptake of pantothenic acid (vitamin B5) and biotin (vitamin B7).

(F-G) Smvt transcription (qRT-PCR) in dissected carcass/fat body of (F) Act88FGal4>UAS-RelishRNAi#1, UAS-GdhRNAi#1 flies or (G) in Dro-GFP flies after P.e. oral infection; n=3.

(H) Smvt transcription (measured by qRT-PCR) in dissected w1118 carcass/fat body after ex vivo incubation with the indicated concentration of glutamate for 5 hrs; n=3.

(I) Smvt transcription (measured by qRT-PCR) in dissected carcass/fat body of CGGal4>UAS-dmGlutRNAi flies after ex vivo incubation with 20mM glutamate for 5 hrs; n=3.

(J-K) Changes in systemic lipid metabolism of CGGal4>UAS-Smvt flies after P.e. oral infection. (J) ORO stain of dissected carcass/fat body. (K) Total TAG levels of whole flies; n=3.

(L) Circulating lipids levels from isolated hemolymph of CGGal4>UAS-Smvt or CGGal4>UAS-SmvtRNAi#1 flies after P.e. oral infection; n=3.

Error bars represent mean±SE, *P<0.01.