| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse polyclonal anti-ATP5A | abcam | ab14748 |
| Mouse polyclonal anti-NDUFS3 | abcam | ab14711 |
| Rabbit polyclonal anti-GLUD1 | Sigma-Aldrich | HPA061369 |
| Rabbit polyclonal Anti-Histone H3 (acetyl K9) | abcam | Ab4441 |
| Rabbit polyclonal Anti-Relish | RayBiotech | RB-14-0004 |
| Rabbit polyclonal anti-beta-actin | Cell Signaling | 4967 |
| Goat Anti-Rabbit IgG HRP-conjugate | BioRad | 1706515 |
| Goat Anti-Mouse IgG HRP-conjugate | BioRad | 1706516 |
| Alexa Flour 488-conjugated Anti-Rabbit IgG | Jackson Immunoresearch | 119191 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa Fluor 555 Phalloidin | Thermo Fisher Scientific | A34055 |
| Alexa Fluor 488 Phalloidin | Thermo Fisher Scientific | A12379 |
| DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) | Thermo Fisher Scientific | D1306 |
| Drosophila Agar, Type II | Genesee | 66-103 |
| Malt Extract | Genesee | 62-110 |
| Inactive Dry yeast | Genesee | 62-106 |
| Cornmeal | Genesee | 62-101 |
| Propionic acid | VWR | TCP0500-500mL |
| Methyl 4-Hydroxybenzoate | VWR | 97061-946 |
| Sucrose | VWR | 97063-788 |
| Trizol | Life Technologies | 15596018 |
| Superscript III Reverse Transcriptase | Life Technologies | 2129278 |
| iTaq Universal SYBR Green Supermix | BioRad | 1725121 |
| Nitrocellulose membrane | VWR | 10600006 |
| Oil Red O | abcam | ab150678 |
| Nile Red | Life Technologies | N1142 |
| Phosphoric acid | VWR | 97064-780 |
| Brilliant blue | Sigma-Aldrich | B0149 |
| ECL Western Blotting Substrate | Pierce | 32106 |
| L-Glutamate | abcam | Ab120049 |
| Biotin | Sigma-Aldrich | B4639,14400 |
| D-Pantothenic acid | Sigma-Aldrich | P5155 |
| L-Ascorbic acid | Sigma-Aldrich | A7506 |
| DreamTaq PCR Master Mix | Thermo Fisher Scientific | K1081 |
| Xho I | New England BioLab | R0146S |
| NotI-HF | New England BioLab | R3189S |
| T4 DNA Ligase | New England BioLab | M0202T |
| RNase A | QIAGEN | 19101 |
| Protease Inhibitor Cocktail | Thermo Fisher Scientfic | 78440 |
| Proteinase K | VWR | 0706 |
| LiCl | Amresco | 0416-100G |
| KCl | J.T.Baker | 3052-01 |
| CaCl2 | Macron | 4160-12 |
| PMSF | Thermo Fisher Scientific | 36978 |
| Bovine Serum Albumin | VWR | 97061 |
| Critical Commercial Assays | ||
| StanBio Liquicolor Triglycerides Kit | Fisher | SB-2100-430 |
| Bio-Rad Protein Assay | Bio-Rad | 5000006 |
| TMRE-Mitochondrial Membrane Potential Assay Kit | abcam | Ab113852 |
| QIAquick PCR purification Kit | QIAGEN | 28104 |
| QIAquick Gel extraction Kit | QIAGEN | 28704 |
| QIAprep spin miniprep Kit | QIAGEN | 27104 |
| Plasmid midi Kit | QIAGEN | 12143 |
| Glutamate Assay Kit | Sigma-Aldrich | MAK004 |
| α-Ketoglutarate Assay Kit | Sigma-Aldrich | MAK054 |
| Deposited Data | ||
| Raw and analyzed RNA sequencing data | This paper | GEO: GSE160652 |
| Experimental Models: Organisms/Strains | ||
| D. melanogaster: w1118 | Bloomington Drosophila Stock Center and (Hazelrigg et al., 1984) | BDSC: 3605; FlyBase: FBst0003605 |
| D. melanogaster: Act88FGal4 (w*; P{Act88F-GAL4.1.3}3) | Bloomington Drosophila Stock Center and (Gajewski and Schulz, 2010) | BDSC: 38461; FlyBase: FBst0038461 |
| D. melanogaster: MhcGal4 (w*; P{Mhc-GAL4.K}2/TM3, Sb1) | Bloomington Drosophila Stock Center and (Klein et al., 2014) | BDSC: 55133; FlyBase: FBst0055133 |
| D. melanogaster: PplGal4 (w*; P{ppl-GAL4.P}2) | Bloomington Drosophila Stock Center and (Zinke et al., 1999) | BDSC: 58768; FlyBase: FBst0058768 |
| D. melanogaster: LppGal4 (w[*]; P{w[+mC]=Lpp-GAL4.B}3) | Bloomington Drosophila Stock Center and (Brankatschk and Eaton, 2010) | BDSC: 84317; FlyBase: FBst0084317 |
| D. melanogaster: tubP-Gal80ts (P{tubP-GAL80ts}20) | Bloomington Drosophila Stock Center and (Ferris et al., 2006) | BDSC: 65406; FlyBase: FBst0065406 |
| D. melanogaster: UAS-Apoltp RNAi (y1sc*v1; P{TRiP.HMC03294}attP2) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC:51937; FlyBase: FBst0051937 |
| D. melanogaster: UAS-Rfabg/Apolpp RNAi (y1v1; P{TRiP.HM05157}attP2) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC:28946; FlyBase: FBst0028946 |
| D. melanogaster: UAS-Rfabg/Apolpp RNAi (y1sc* v1; P{TRiP.HMS00265}attP2/TM3) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC:33388; FlyBase: FBst0033388 |
| D. melanogaster: UAS-ATPsynbeta RNAi (y [1] v [1]; P{y[+t7.7] v[+t1.8] =TRiP.JF02892} attP2) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC: 28056; FlyBase:FBgn0010217 |
| D. melanogaster: UAS-luciferase RNAi (y1v1; P{TRiP.JF01355}attP2) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC: 31603; FlyBase: FBst0031603 |
| D. melanogaster: UAS-Gdh RNAi (y [1] sc[*] v[1] sev[21]; P{y[+t7.7] v[+t1.8]=TRiP.GLC01815}attP2) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC: 53255; FlyBase:FBgn0001098 |
| D. melanogaster: UAS-Gdh (y [1] w[67c23]; P{y[+mDint2] w[+mC] =EPgy2} Gdh[EY07150]) | Bloomington Drosophila Stock Center and (Bellen et al., 2004) | BDSC: 20165; FlyBase:FBgn0001098 |
| D. melanogaster: UAS-dmGlut RNAi (y1 sc* v1 sev21; P{TRiP.HMS01615}attP2/TM3, Sb) | Bloomington Drosophila Stock Center and (Perkins et al., 2015) | BDSC: 36724; FlyBase:FBgn0010497 |
| D. melanogaster: UAS-Rel RNAi (w1118; P{GD1199} v49413) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 49413; FlyBase: FBst0468440 |
| D. melanogaster: UAS-Rel RNAi (w1118; P{KK109851} VIE-260B) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 108469; FlyBase: FBst0480279 |
| D. melanogaster: UAS-Key RNAi (w1118; P{GD1249} v7723) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 7723; FlyBase: FBst0470808 |
| D. melanogaster: UAS-Dredd RNAi (w1118; P{KK110428} VIE-260B) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 104726; FlyBase: FBst0476565 |
| D. melanogaster: UAS-PGRP-LC RNAi (w1118; P{KK105287} VIE-260B) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 101636; FlyBase: FBst0473509 |
| D. melanogaster: UAS-PGRP-LE RNAi (w1118; P{GD14089} v23664) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 23664; FlyBase: FBst0455134 |
| D. melanogaster: UAS-White RNAi (GD) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 30033; FlyBase: FBgn0026792 |
| D. melanogaster: UAS-Gdh RNAi (w1118; P{GD11605} v22059) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 22059; FlyBase:FBgn0001098 |
| D. melanogaster: UAS-Smvt RNAi (w1118; P{KK105033} VIE-260B) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 102662; FlyBase:FBgn0039873 |
| D. melanogaster: UAS-Smvt RNAi (w1118; P{GD12439} v40650/TM3) | Vienna Drosophila RNAi Center and (Dietzl et al., 2007) | VDRC: 40650; FlyBase:FBgn0039873 |
| D. melanogaster: UAS-Smvt | This paper | N/A |
| D. melanogaster: UAS-LD-GFP | (Yu et al., 2011) | N/A |
| D. melanogaster: CGGal4 (w*; P{CG-GAL4.A}) | (Hennig et al., 2006) | N/A |
| D. melanogaster: TubGSGal4 (y1w*; TubGeneSwitch/CyO) | (Sykiotis and Bohmann, 2008) | N/A |
| D. melanogaster: Drosocin-GFP | (Chatterjee et al., 2016b) | N/A |
| D. melanogaster: UAS-Rpr | (Aplin and Kaufman, 1997) | N/A |
| D. melanogaster: Hml∆G4, UAS-GFP | (Sinenko and Mathey-Prevot, 2004) | N/A |
| Oligonucleotides | ||
| Primers for Dipt, see Table S1 | This paper | N/A |
| Primers for Dro, see Table S1 | This paper | N/A |
| Primers for AttA, see Table S1 | This paper | N/A |
| Primers for CecA, see Table S1 | This paper | N/A |
| Primers for Gdh, see Table S1 | This paper | N/A |
| Primers for Smvt, see Table S1 | This paper | N/A |
| Primers for Actin 5c, see Table S1 | This paper | N/A |
| Primers for P. entomophila, see Table S1 | This paper | N/A |
| Primers for Erwinia carotovora carotovora15, see Table S1 | This paper | N/A |
| Primers for Smvt-pUAST, see Table S1 | This paper | N/A |
| Primers for R1 (Gdh locus), see Table S1 | This paper | N/A |
| Primers for R2 (Gdh locus), see Table S1 | This paper | N/A |
| Primers for R3 (Gdh locus), see Table S1 | This paper | N/A |
| Primers for R4 (Gdh locus), see Table S1 | This paper | N/A |
| Primers for R5 (Gdh locus), see Table S1 | This paper | N/A |
| Primers for Act5cP (promoter region), see Table S1 | This paper | N/A |
| Primers for DroP (promoter region), see Table S1 | This paper | N/A |
| Primers for DiptP (promoter region), see Table S1 | This paper | N/A |
| Drosophila Negative Control (NC) Primer Set 1 | Active Motif | 71028 |
| Software and Algorithms | ||
| ImageJ | NIH Image | https://imagej.net/ImageJ |
| Heatmapper | University of Alberta | http://www.heatmapper.ca/expression/ |
| FlyMine | Cambridge University | https://www.flymine.org//flymine/begin.do |
| FlyBase | N/A | https://flybase.org/ |
| DNA Sequencing | Eton Bioscience | https://www.etonbio.com/ |
| Generation of Transgenic Flies | Rainbow Transgenic Flies | https://www.rainbowgene.com/ |
| JASPAR Database | N/A | http://jaspar.genereg.net/ |
| Other | ||
| Illumina HiSeq 2500 | Illumina | N/A |
| Leica M165FC system | Leica | N/A |
| StepOnePlus Real-Time PCR systems | Applied Biosystems | N/A |
| Nikon Eclipse Ti confocal system | Nikon | N/A |
| Capillaries | VWR | 53432-706 |
| Bioruptor/sonicator | Diagenode | UCD-200 |
| Pierce Protein A magnetic beads | Thermo Fisher Scientific | 88845 |
| QUBIT 4 Fluorometer | Thermo-Fisher Scientific | N/A |
| Epoch Microplate | BioTek | N/A |
| Ecc15 Bacteria | Basset et al., 2000 | N/A |
| Pseudomonas entomophila (P.e.) | Liehl et al., 2006 | N/A |
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