Fig. 1.

Nutrient stress suppresses BMP signaling. A Fluorescence quantification of C. elegans expression BMP transcriptional reporter spp-9::GFP after 8 h of starvation (n = 30 animals). B Fluorescence quantification of C. elegans BMP transcriptional reporter spp-9::GFP treated with 50 and 4.5 µM Metformin and Phenformin, respectively (n = 30 animals). Drugs were included in media for 3 days post-egg laying. C Western blot analysis of starved A549 cells with quantification. D Immunofluorescence image and quantification for BMPR2 of starved A549 cells. FCS indicates fetal calf serum. DPBS indicates Dulbecco’s phosphate buffered saline