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Quantification of binding of probe XAN818 relative to that of probe EUB338 measured as fluorescence intensity. Different stringencies were obtained by varying the concentration of formamide in the hybridization buffer. The target sequence of probe XAN818 and base changes in nontarget organisms are shown in Table 3. Symbols: ●, S. nitritireducens DSM 12575T; ◊, B. alba DSM 1416; □, A. acidophilum DSM 700T; ▴, G. oxydans subsp. oxydans DSM 3503T. Error bars show standard deviations.
