Fig. 2.

Effect of SL on viability and cytokines levels in DNP-IgE/HSA-sensitized RBL-2H3 mast cells. A Cell viability was assessed using the MTT assay in RBL-2H3 cells treated with anti-DNP-IgE (110 ng/ml) for 1 h, followed by stimulation with SL (5%) for 24 h. The mRNA expression of cytokines, B IL-4, C IL-5, and D IL-13, was evaluated using qPCR in DNP-IgE/HSA induced RBL-2H3 mast cell. Each gene expression was measured in RBL-2H3 cells treated with anti-DNP-IgE (110 ng/ml) for 1 h, followed by stimulation with SL (5%) for IL-4 and IL-13 for 1 h, and IL-5 (3 h), and then with 25 ng/mL DNP-HSA antigen (25 ng/ml). Gene expression was normalized with GAPDH. Data are presented as the mean ± SEM (n = 3). Statistical significance was determined by one-way analysis of variance according to Student’s t-test; **p < 0.01, ***p < 0.001 vs. non-treated control