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Application of s-urine solutions. Six skin sites were marked on both volar forearms (a), and HillTop chambers were impregnated with 500 µL s-urine and applied to the skin using a medical tape to keep them in place (b). A sixth site served as the untreated control. c Prior to each treatment and after exposure, TEWL was measured to assess skin barrier disruption with the Tewameter® TM 300.
