Astrogliogenesis in the SVZ. (A–D) Representative brain images of control (Ctrl), ethanol (EtOH), cocaine (Coc), and combination (Combo) treated mice, respectively, immunostained with stem cell marker (GFP, green), astrocyte marker (GFAP, red), and merged with nuclear maker DAPI (Bregma 0.5 through 1.08). (E–G) Quantification of GFAP+, GFAP+GFP+, and the percentage of GFAP+GFP+ over total GFP+ cells in the SVZ and origin of the rostral migratory stream (area inside red square). Values are shown as mean ± SEM, *p < 0.05 compared to control, #p < 0.05 compared to other sex in the same group, $p < 0.05 compared to cocaine group, +p < 0.05 compared to combination group, n = 3 mice per sex per group. Scale bars, 45 μm.