Neural stem cell and neurogenesis in the subgranular zone (SGZ). (A–D) Representative brain images of control (Ctrl), ethanol (EtOH), cocaine (Coc), and combination (Combo) treated mice, respectively, stained with stem cell marker (GFP green), neuronal marker (DCX red), and merged with nuclear maker DAPI (Bregma 0.5 through 1.08). White boxes represent increased magnification of selected cells as seen on the right side of panel. (E–H) Quantification of GFP+, DCX+, DCX+GFP+, and the percentage of DCX+GFP+ over total GFP+ cells, in the SGZ. Values are shown as mean ± SEM, *p < 0.05 compared to control, #p < 0.05 compared to other sex in the same group, $p < 0.05 compared to cocaine group, +p < 0.05 compared to combination group, n = 3 mice per sex per group. Scale bars, 45 μm.