Figure 4.

Suppression of ferroptosis and buffering of Fe²⁺ accumulation alleviate proliferation inhibition and invasion inhibition of ccRCC induced by Lut. (a) Results of the CCK-8 assay in 786-O and OS-RC-2 cells, respectively. (b) Morphological changes of 786-O cells treated under different conditions (magnification, ×40). (c) Phalloidin staining of 786-O cells treated under different conditions (magnification, ×400). (d) Proliferative ability of 786-O cells treated under different conditions displayed by the EdU assay (magnification, ×200). (e) Semiquantification of EDU in (d). Representative images (f) and semiquantitative analyses (g) of 786-O and OS-RC-2 cells according to Transwell invasion assays (magnification, ×200). The data in (a), (e), and (g) are expressed as the mean ± SD. ^∗P < 0.05 vs. the NC group, ^#P < 0.05 vs. Lut group. EdU: 5-ethynyl-2′-deoxyuridine; Lut: luteolin; DFP: deferiprone; Fer-1: ferrostatin-1.