Figure 1:

Decision tree for the assignment of genotype and activity score (AS). Each maternal DNA sample was analyzed using real-time PCR and TaqMan genotyping assays for *2, *3, *4, *6, *9, *10, *17, *35, and *41 alleles. A copy number assay was also performed and a genotype of *5 was assigned if only 1 allele was determined to be present. A *1 was assigned for alleles negative for all assays lacking *2 allele SNP. White boxes represent the assay performed for a given allele, grey diamonds represent the resulting allele assigned, and the grey ovals represent the activity score assigned to the corresponding allele.