Table 1.
HydG rates for free CO formation as monitored via single wavelength kinetics experiments (λ = 425 nm, see Fig. 2)
| Enzyme | Burst phasee (min−1) | Biphasic, rate 1 f (min−1) | Biphasic, rate 2 f (min−1) |
|---|---|---|---|
|
| |||
| HydGΔEF a | 0.0836 ± 0.0022 | 0.0658 ± 0.0034 | 0.0370 ± 0.0079 |
| HydGEF b | 0.0973 ± 0.0193 | 0.0637 ± 0.0046 | 0.0348 ± 0.0052 |
| Dangler loaded HydGΔEF c | 0.0941 ± 0.0036 | 0.0640 ± 0.0001 | 0.0529 ± 0.0032 |
| H272A HydGΔEF d | 0.0036 ± 0.0001 | N/A | N/A |
a
WT traditionally reconstituted HydGΔEF with 7.54 ± 0.48 Fe per protein.
b
WT traditionally reconstituted HydGEF with 7.38 ± 0.40 Fe per protein.
c
WT dangler reconstituted HydGΔEF with 8.23 ± 0.43 Fe per protein.
d
Traditionally reconstituted H272A HydGΔEF with 7.2 ± 0.4 Fe per protein. N/A = not applicable.
e
Burst phase rate determined by linear fit to data between 0–5 min.
f
These rates come from biphasic exponential fits to the same data, using all data points from 0 to 120 min.