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. 2022 May 26;11:giac045. doi: 10.1093/gigascience/giac045

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© The Author(s) 2022. Published by Oxford University Press GigaScience.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — An X-Y/scatterplot using normalized counts of subgenomic mRNAs (sgmRNAs) (with >5 A residues at the 3′ end—indicative of a polyA tail for the direct RNA sequencing [dRNAseq] data). To generate the scatterplots, Nanopore dRNAseq data were plotted against the either the normalized count (at least one primer sequence) of sgmRNAs with (A) ARTIC-Nanopore sequencing data and (C) ARTIC-Illumina sequencing data or provided as ratio (B) and (D), respectively, for S:orf3:E:M:orf6:orf7a:orf7b:orf8:N:orf10 (using data from Supplementary Tables 3, 4, and 5).