Fig 1. RVFV infects microglia.

Microglia cell lines EOC 13.31 and SIM A9 and primary microglia derived from the brains of neonatal mice, along with Vero positive control cells, were infected with RVFV MP-12. The percentage of cells positive for RVFV was assessed by flow cytometry. Representative plots of Vero cell infection analysis (A). RVFV infection rate for all cell types (B). Primary microglia derived from wildtype (WT) mice were infected with RVFV MP-12 or ZH501 and the percentage of cells positive for RVFV was assessed by flow cytometry (C). The relative expression of 40 antiviral response genes with the highest fold change in infected versus uninfected cells (D). Data in (B-C) are shown as the mean +/- SD. Data in (D) and (E) are shown as the mean log₂ fold change in infected versus uninfected cells +/- SEM. *denotes significant changes in gene expression beyond 95% CI between uninfected vs infected cells.