Fig 6. Microglia from Mavs^-/- brains have defective response to RVFV infection.

UMAP plots depicting cell clusters of myeloid lineage cells derived from WT and Mavs^-/- brains, +/- RVFV infection. Cell types (A) or conditions (B) are color coded. Gene markers for specific cell types (C). The distribution of myeloid lineage cell types within brains of each condition (D). Expression of type I IFNs within microglia (clusters 0 and 1) (E). Gene ontology (GO) enrichment analysis showing pathways enriched in WT vs Mavs^-/-, or Mavs^-/- vs WT microglia (clusters 0 and 1) from infected brains are shown in (F) and (G), respectively. Violin plots depicting the relative expression of selected genes within microglia (clusters 0 and 1) are shown in (H). Flow cytometry: microglia were defined as CD11b^int CD45^int as described in Fig 5, and the percentage of RVFV⁺ microglia are shown in (I). Mean fluorescence intensities (MFI) of CD86 and I-A/I-E expression on microglia (J) and (K), respectively. Data in (I-K) are shown as the mean +/- SD. **p<0.01, ***p<0.001, ****p<0.0001.