Fig 2. Genetically diverse and previously undescribed lineages of pathogenic Leptospira are widespread in Puerto Rico.

A) Maximum likelihood phylogeny constructed using a 202bp segment of the lipL32 gene, including sequences obtained from soil and water samples collected in this study (blue text) and those available in public databases (black text). Nine major genetic clades are represented, seven of which were present in Puerto Rico, including four not previously described. Clades are labelled 1 through 9 on the tree and color coded. B) Sites where each major clade was collected, with colors within the pie charts corresponding to those in the phylogeny; superscript indicates if soil (S) and/or water (W) samples were positive at each site. Fifteen positive sites are included on this map. This map was created using ArcGIS software by Esri. ArcGIS and ArcMap are the intellectual property of Esri and are used herein under license. Copyright Esri. All rights reserved. For more information about Esri software, please visit www.esri.com. Basemap: http://goto.arcgisonline.com/maps/Canvas/World_Light_Gray_Base.