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. 2022 Jan 10;25(5):412–424. doi: 10.1093/ijnp/pyac004

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© The Author(s) 2022. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Expression of TMEFF1 in response to incubation of morphine craving. (A, B) The subcellular localization of TMEFF1 by using the laser scanning microscope. The TMEFF1 protein labeled with green fluorescence, A: neuron nuclei (NeuN), and B: astrocytes (GFAP) labeled with red fluorescence (Scale bar = 75 μm). (C) The expression of TMEFF1 mRNA between T1 and T2 in NAcC or NAcSh. (D, E) Western-blot analysis of TMEFF1 and GAPDH expression between T1 and T2 in NAcC or NAcSh. **P < .01, vs T1 in NAcC. (F) The expression of TMEFF1 mRNA between WD1 and WD2 in NAcC or NAcSh without test. (G, H) Western-blot analysis of TMEFF1 and GAPDH expression between WD1 and WD2 in NAcC or NAcSh without the test.