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. 2022 Jan 10;25(5):412–424. doi: 10.1093/ijnp/pyac004

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© The Author(s) 2022. Published by Oxford University Press on behalf of CINP.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The expression of TMEFF1 was regulated by miR-592-3p in the NAcC. (A) The predicted miR-592-3p binding site in the 3′-UTR region of TMEFF1 and the designed mutant sequences of TMEFF1 were indicated. (B, C) Relative luciferase activities of miR-592-3p were measured in the 293T cells that co-transfected TMEFF1 with wild-type luciferase vector or mutant luciferase vector, ** P < .01 vs mimics control, * P < .05 vs inhibitor control. (D) The expression of miR-592-3p was significantly increased after injection of rAAV-miR-592-3p (rAAV-TRE-CMV-EGFP-miR-592-3p-WPRE-hGHpA), ***P < .001 vs control or rAAV-vector (n = 4). (E) The expression of TMEFF1 was significantly decreased after injection of the upregulated rAAV-miR-592-3p during the incubation of morphine craving. **P < .01 vs control or rAAV-vector (n = 6). (F) The expression of miR-592-3p was significantly decreased after injection of rAAV-Decoy-miR-592-3p (rAAV-TRE-U6-Decoy RNA (miR-592-3p)-EGFP-SV40-pA), ***P < .001 vs control or rAAV-vector (n = 4). (G) The expression of TMEFF1 was significantly increased after injection of down-regulation rAAV-Decoy-miR-592-3p during the incubation of morphine craving. ***P < .001 vs control or rAAV-vector (n = 6).