Figure 4.
Cell viability was affected by the presence of VEA in Δ8THC and menthol flavoring in nicotine-based e-liquids especially at 5 V. Acute in vitro cell viability assessment, using LDH assay, following exposure to e-cigs aerosols generated from different concentrations of THC/VEA or nicotine-based e-liquids, under 2 operational voltages (3.7 or 5 V), or cigarette smoke, at 2 doses of 85 and 257 puffs/m2 (1 and 3 months), on human lung epithelial cells (Calu-3) (A, C) and peripheral monocytes (THP-1) (B, D). All values are represented as the mean± SE. * p < .05, values statistically different from untreated cells. # p < .05, values statistically different between 2 doses. Statistical analysis was performed on an average of 3 independent biological samples; fold changes >20% were deemed to be statistically significant (p < .05).